【摘要】 采用紫外线、He -Ne激光及紫外线与He-Ne激光复合作用的方法,对本实验室的一株产PHB的细菌G -Q7进行诱变,以提高其产量 结果表明,紫外线和He -Ne激光单独作用,诱变效果不佳,而二者的复合作用却能产生很好的效果 用He- Ne激光辐照20min再经紫外线照射150s,正变菌株的细胞量为11. 5g/L,PHB含量为65%,并具有良好的遗传稳定性 对诱变前后菌株积累的PHB产物的外观、热性质和13C- NMR图谱进行分析,结果表明该诱变使代谢途径中的某种酶发生了改变。更多还原

【Abstract】 The strain G-Q7 producing PHB was irradiated respectively by UV, He-Ne laser and both of them to increase PHB production. The results indicated that the coordinative effect of UV and He-Ne laser was better than using them individually. The cells were treated firstly by He-Ne laser for 20 minutes and then by UV for 150 seconds, which resulted in a final cell concentration of 11.5 g/L, PHB content of 65%. The mutant had better hereditary stability. The two PHB polymers obtained from the strains G-Q7 and the mutant were analysed by the method of DSC and NMR, the conclusion appears that the mutagenic effect makes some certain enzyme of metabolism change.更多还原