【摘要】 研究了Alcalase水解猪皮制备胶原蛋白寡肽时影响水解效果的各种因素。确定了Alcalase水解猪皮的最佳水解条件:pH值8.0,水解温度60℃,酶与底物比25μL/g,底物浓度60g/L,水解时间4h。在此最佳水解条件下,猪皮的降解率可达到97.3%,水解度可达到13.1%。更多还原

【Abstract】 Alcalase was used to hydrolyze pigskin, and the factors effecting pigskin hydrolysate harvest, such as the amount of Alcalase , temperature, pH and time of hydrolyzing, were studied. The best conditions of the hydrolysis of pigskin were: pH8.0, hydrolyzing temperature 60 ℃ ,ratio of Alcalase 25μL/g, concentration of substrate 60g/L, total hydrolysis time 4h. Under these conditions the degradation percentage of the pigskin can reach 97.3%, while the hydrolysis degree of collagen protein (i.e., the ratio of the peptide bonds cleavaged/total peptide bonds in protein) can reach 13.1%更多还原