【摘要】 利用生物信息学手段,在GenBank数据库进行氨基酸的同源性检索分析,发现来自谷氨酸棒杆菌(Corynebacteriumglutamicum)一功能未确定的ORF序列被注释为假设的海藻糖酶(putativetrehalosesynthase),它与已报道的海藻糖合成酶的氨基酸序列有60%以上的同源性。本研究把这段ORF克隆到大肠杆菌进行表达及进行功能鉴定。实验表明这段ORF序列为一新的海藻糖合成酶基因,其表达产物能将麦芽糖分子转化成海藻糖分子。重组酶性质的初步研究表明重组酶在pH7.0~7.5,30℃转化麦芽糖效率最高。更多还原

【Abstract】 An open reading frame (ORF) was annotated as putative trehalose synthase in Corynebacterium glutamicum sequenced genome, which has been found in GenBank database through amino homology blast base on combination of bioinformatics. Blasting against the database indicated that amino acid sequence of this ORF had less than 60% homology with the reported terhalose synthases. The function, which was identified by expressing this ORF in Escherichia coli, proved this ORF was a novel trehalose synthase gene. Recombinant enzyme showed the optimal activity was at pH 7.07.5 and 30 when it converted maltose into trehalose.更多还原