【摘要】 目的探讨树突状细胞(DC)对肿瘤浸润淋巴细胞(TIL)和小鼠脾淋巴细胞(小鼠脾LC)的最佳激活作用比例。方法联合应用GM-CSF、IL-4和小鼠H22肝癌细胞全细胞性抗原致敏从荷瘤小鼠四肢长骨提取的DC,依据不同的激活比例用致敏DC体外激活TIL和小鼠脾LC,采用乳酸脱氢酶(LDH)4小时释放法测定、观察被不同程度激活的TIL和小鼠脾LC对小鼠H22肝癌细胞的杀伤活性。结果当E/T为1:400和1:200时,所激活的TIL或小鼠脾LC的杀伤活性较弱,线性关系不明显,P>0.05;当E/T为1:100时,杀伤活性有较明显的提高,与前两者比较,P<0.05;当E/T=1:50时,杀伤活性徒增,与前三者比较均有显著性差异;而当E/T=1:25,1:12.5和1:6.25时,杀伤活性与E/T=1:50时基本没变化,P>0.05。结论当E/T=1:50时,DC能使小鼠脾LC和TIL充分激活,显著提高其对小鼠H22肝癌细胞的体外杀伤活性。更多还原

【Abstract】 Ojective To investigate the optimal rate of effect cells/target cells(E/T)of tumour infiltrating lymphocytes(TIL)and splenocyte lymphocytes stimulated by dendritic cells(DC).Methods DC were isolated from mouse bone marrow and stimulated by granulocyte/macrophage colony stimulating factor(GM-CSF),inˉterleukin-4(IL-4)and tumor antigen.Then TILand splenocyte lymphocytes were stimulated by DC in different E/Tconditions,and their killing activity on H22cells in vitro were observed respectively.Result At E/Tof1:400and1:200,the killing rates of TIL and splenocyte lymphocytes stimulated by DC were low,E/Tof1:100,the killing rates of TIL and splenocyte lymphocytes stimulated by DC were higher than the first two groups(P<0.05);At E/Tof1:50,the killing rates of TIL and splenocyte lymphocytes stimulated by DC were obviously increased;but E/Tof1:25,1:12.5and1:6.25,the killing rates of TIL and splenocyte lymphocytes stimulated by DC didn’t have any change to compare with that of E/Tof1:50(P>0.05).Conclusion At E/T of1:50TIL and splenocyte lymphocytes can be stimulated by DC efficiently,and their killing rates against H22were sigˉnificantly higher than those not stimulated by DC.更多还原