【摘要】 目的:检测FHIT基因mRNA在良、恶性胸腔积液中的表达水平。方法:采用巢式逆转录-聚合酶链反应(RT-PCR)方法对40例良、恶性胸腔积液进行FHIT基因mRNA表达的检测。随机抽取3例FHIT基因的RT-PCR扩增产物的目的片段进行DNA序列测定。结果:22例恶性胸腔积液标本中FHIT基因缺失或异常转录的表达率为68.2%(15/22)。18例良性胸腔积液中FHIT基因均正常表达。FHIT基因mRNA在良、恶性胸腔积液中的表达差异显著(P<0.01)。测序结果表明RT-PCR产物确为FHIT基因。结论:胸腔积液中FHIT基因mRNA的检测有望成为鉴别诊断良、恶性胸腔积液的重要方法。更多还原

【Abstract】 Objective:To detect abnormal expressions of fragile histide traid(FHIT) in benign and malignant pleural effusion at mRNA level.Methods:Using a nested reverse transcription-polymerase chain reaction assay (Nested RT-PCR), the expressions of FHIT in 40 benign and malignant pleural effusion samples were examined. 3 samples selected randomly from each FHIT PCR positive results were sequenced.Result:Of the 22 malignant pleural effusion samples , the FHIT was expressed at mRNA level in 68.2%(15/22).Of the 18 benign pleural effusion samples, none of abnormal expression in FHIT. There was significant difference of the frequency in FHIT gene expression was found between benign and malignant pleural effusion (P<0.01). The DNA sequence confirms that the RT-PCR products were truly FHIT cDNA.Conclusion:Detection of FHIT can be a promising diagnostic method in differential diagnoses of benign and malignant pleural effusion .更多还原