【摘要】 目的 :研究广西巴马地区长寿老人载脂蛋白 E(Apo E)基因多态性与巴马长寿现象的关系。方法 :采用聚合酶链反应 -限制性片段长度多态性 (PCR- RFL P)技术 ,对 12 9名年龄 90～ 113岁的广西巴马地区长寿老人和 30 7名作为相应年龄对照的长寿家庭成员 ,并以当地非长寿自然人群 15 3人作为对照组 ,进行 Apo E基因分型。分别比较长寿老人与各组人群的基因型、基因频率的分布特征。结果 :1广西巴马地区长寿老人 Apo E基因型频率中以ε3/3最高 ,其次是ε2 /3和ε3/4,而ε2 /4和ε4 /4频率最少。长寿老人组与其亲属年龄对照的 Apo E基因型和基因频率相比之间均无显著性差别 ;2长寿组与当地自然人群对照相比呈显著性阳性关联 (P =0 .0 0 1,P =0 .0 4 )的为ε2 /ε2 (5 .34% ;0 % )和ε3/ε3(79.39% ;6 8.6 3% )基因型。这两种基因型携带者在长寿人群中占 85 %以上。结论 :Apo E基因多态性与广西巴马长寿现象有关联。巴马地区长寿相关基因为 Apo Eε2 /ε2、ε3/ε3基因型。其中ε3/ε3纯合子是最主要的长寿关联基因。更多还原

【Abstract】 Objective:To study the relation between apolipoprotein E (apoE) allele and longevity in Bama area in Guangxi,China.Methods:The apoE genotypes were analyzed with PCR-RFLP in 129 persons of 94-113 years old,307 persons from the longevity families,and the local residents 153 persons with ages ranging 60-80 years old as control group. We compared the differences of allele, genotype of longevity group with the other groups.Result:①The ApoE ε3/ε3 genotypes have highest frequency in long-lived elderly in Bama area in Guangxi, next isε2/3 and ε3/4,ε2/4 and ε4/4 are lowest in frequency.There were no significant differences of genotype and allele frequencies between longevity and its age matched groups.②There were significant positive associations(P=0.001, P=0.04)for genotypes ε2/ε2(5.34%;0%)and ε3/ε3(79.39%;68.63%), through comparing longevity group with local control group.Conclusion:We find out and confirm the ApoE ε2/ε2 and ε3/ε3 genotypes are associated with longevity in the population in Bama area in Guangxi in China, especially homozygote ε3/ε3 genotype which is perhaps the major gene associated with longevity.更多还原