【摘要】 在弱酸性条件下,阿莫西林能与亚甲蓝(MB)反应生成易被1,2-二氯乙烷萃取的离子对缔合物,其最大吸收波长为λmax=657 nm。据此建立了测定阿莫西林的萃取分光光度法,药物浓度在0.4~6.8 mg.L-1范围内符合比尔定律,在最大吸收波长657 nm处表观摩尔吸光系数ε=5.0×104L.mol-1.cm-1,最低检出限为0.01 mg.L-1,回收率为97.5%~101.3%。实验表明该法可成功地用于药物制剂及尿样中阿莫西林含量的测定,结果令人满意。更多还原

【Abstract】 A method of extractive spectrophotometry has been proposed for the determination of amoxicillin（AMO）.The ion pairs complex of amoxicillin with methylene blue（MB） can be extracted into 1,2-dichloroethane at pH 3.3 and has absorption maximum at 657 nm.The ratio of amoxicillin to methylene blue in the ion pairs complex is 1∶1.The apparent molar obsorptivity of ion pairs complex is 5.0·10⁴ L·mol^-1·cm^-1 at 657 nm.The calibration graph generated is linear over the range of 0.4-6.8 mg·L^-1 drug in solution with a correlation of 0.998 9.The detection limit is 0.01 mg·L^-1 and the recovery of AMO is 97.5%-101.3%.The present method has been applied to the determination of amoxicillin in pharmaceutical formulations and urine samples with satisfactory results.更多还原