【摘要】 利用紫外-可见(UV/Vis)光谱法研究了小分子染料灿烂甲酚蓝(BCB)与小牛胸腺DNA(ct DNA)的相互作用。实验表明,DNA在低浓度时,BCB能结合DNA形成1∶1的化合物,结合常数为4.3×104L.mol-1,DNA与BCB的主要结合方式是“静电作用”方式;而在较高浓度时,则变成“嵌入作用”方式。为了进一步证明BCB分子嵌入到DNA,利用了羟丙基-β环糊精(HP--βCD)与BCB的包合行为来研究。实验证明,BCB分子能进入HP--βCD的疏水性空腔形成包合物,稳定常数为1.98×103L.mol-1;高浓度DNA存在时,BCB分子从HP--βCD空腔中离解而与DNA发生嵌入作用。据此可推断出BCB分子能嵌入到DNA的双螺旋沟槽中。BCB分子属经典的嵌入剂。更多还原

【Abstract】 The interaction of dye brilliant cresyl blue（BCB）,which features a phenoxonium group,with hydroxypropyl-β-cyclodextrinl and DNA in aqueous solution was studied by spectroscopic methods.The experimental results indicate that BCB can be included into the hydrophobic cavity of HP-β-CD to form an inclusion,a complex with a stability constant of 1.98×10³ L·mol^-1.At low DNA concentrations,BCB can be bound to DNA to form a 1∶1 association complex with the binding constant of 4.3×10⁴ mol·L^-1,and the major binding model of BCB to DNA is "electrostatic binding".However,the major binding model changes into "intercalative binding" at high DNA concentrations.In addition,the interactions of BCB-（HP-β-CD） inclusion complex and DNA have been investigated.The results suggest that BCB displayed a high affinity for DNA and the inclusion complex decomposed when it was bound to DNA.更多还原