【摘要】 建立了原花青素的流动注射分析方法。利用原花青素在强酸介质中,铁离子的催化下形成红色花色素,以NH4Fe(SO4)2、盐酸、乙醇的混合溶液为载流,用流动注射-二极管阵列检测器扫描待测物的吸收光谱图,测定最大吸收波长(545 nm)处的吸光度值。优化了影响显色反应的有关因素及流动注射参数,原花青素的浓度在0.010~0.20 mg.mL-1的范围内呈良好的线性关系。方法检出限为5μg.mL-1,加标回收率为82.47%~98.18%,相对标准差1.5%~4.9%。本文所建立的分析方法灵敏、准确,采样频率为10 samples.h-1,并已成功用于保健食品中原花青素的检测。更多还原

【Abstract】 A flow-injection-CCD array detection spectrophotometry for the determination of oligomeric proanthocyanidin（OPC） in health foods was developed in the present paper,based on the reaction that OPC can form red anthocyanidin in hydrochloric acid medium with ferric ion as a catalytic agent.The absorption spectrum of OPC was scanned and the absorbance at 545 nm was determined by flow injection with CCD-array detector.The influencing factors of the chromogenic reaction and parameters of the flow injection analysis were optimized.There is a good linear relationship between the absorbances and the concentrations of OPC in the range of 0.010-0.20 mg·mL^-1.The detection limit of the method was 5 μg·mL^-1.The recoveries were 82.47%-98.18% with the relative standard deviation（RSD） of 1.5%-4.9%.The proposed method is sensitive,accurate and rapid with the sampling frequency of 10 samples·h^-1 and suitable for the determination of OPC in the health food.更多还原