【摘要】 用荧光光谱和紫外可见吸收光谱法研究了在不同温度下,新合成的过硒二苯2,2’二甲酸(PSD)对牛血清白蛋白(BSA)作用的荧光猝灭光谱行为。分别用SternVolmer方程和LineweaverBurk双倒数方程处理试验数据,证实在试验浓度和温度范围内,其荧光猝灭作用更符合静态猝灭作用特征,PSD与BSA可反应并结合形成具有一定结构的复合物,得到了反应的结合常量、结合热力学性质和结合位点等参数;同时探讨了静态猝灭作用的机理和结合力的性质,为了解PSD与BSA的结合方式、在人体内的传输机理和药理作用提供科学依据。更多还原

【Abstract】 The binding of newly compounded perseleno diphenyl 2,2’-diformic acid to bovine serum albumin(BSA) was studied at different temperatures using fluorescence spectrum and UV spectrum. The fluorescence quenching data was analyzed according to Stern-Volmer equation and Lineweaver-Burk double-reciprocal equation. It was showed that this quenching complies better with the charactristic of static fluorescence quenching. The binding constant, thermodynamic parameters, and the binding spot of the compound with certain structure, coming from perseleno diphenyl 2,2’-diformic acid and bovine serum albumin, were obtained. Besides, the mechanism of static fluorescence quenching and the quality of binding power were both discussed. The information of the binding mode, the mechanism of its transportation, and some medical theories in human body were offered.更多还原