【摘要】 针对微生化芯片样品剂量少和与微流体芯片集成等要求,设计了两种在芯片内用分光光度法对混合后液体的吸收光谱进行探测的方法。实验利用5 10～84 0nm的可见光光源,采用了直接探测法和消逝波探测法对样品进行探测。通过两种方法的比较证明:直接探测法具有原理简单、消耗样品量少和结构容易实现等优点;消逝波探测法具有需要样品量极少、灵敏度高和易于集成的优点。更多还原

【Abstract】 For the need of biochemical chip, which consumes fewer specimens and is easy to integrate with micro-fluid chip, two kinds of spectrophotometric analysis methods are described in the present paper. Both the direct detection method and evanescent wave detection method are used in the experiments with visible light(460-800 nm). The experimental results proved that the direct detection is simple and evident; on the other hand the evanescent wave detection method consumes much less reagent and is easy to integrat with microchips.更多还原