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水分胁迫下马铃薯试管苗的生理响应

Physiological response of test-tube plantlets of potato under water stress

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【作者】 李建武王蒂司怀军彭晓莉

【Author】 LI Jian-wu1, 2,WANG Di1, 2,SI Huai-jun3,PENG Xiao-li1 (1. College of Agronomy, Gansu Agricultural University, Gansu, Lanzhou 730070;2. Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Gansu, Lanzhou 730070; 3. College of Life Science, Gansu Agricultural University, Gansu, Lanzhou 730070, China)

【机构】 甘肃农业大学农学院甘肃农业大学生命科学技术学院甘肃农业大学农学院 甘肃兰州730070 甘肃省作物遗传改良与种质创新重点试验室甘肃农业大学甘肃兰州730070甘肃兰州730070 甘肃省作物遗传改良与种质创新重点试验室甘肃兰州730070

【摘要】 利用PEG-6000模拟根际水分胁迫法,对马铃薯试管苗进行了水分胁迫处理,研究马铃薯不同品种在不同水分胁迫程度下的生理反应。结果表明,马铃薯试管苗体内丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性与可溶性蛋白质含量在水分胁迫下发生了不同的变化。其中,在轻度水分胁迫(10%PEG)下各马铃薯品种可溶性蛋白含量均上升,脯氨酸大量积累,MDA含量急剧增加,POD、SOD、CAT活性上升;经重度水分胁迫(20%PEG)后,以上各指标变化趋势不一。马铃薯不同品种对水分胁迫的反应敏感程度不一致,这与其不同的抗旱能力有关。

【Abstract】 Test-tube plantlets of potato were treated with water stress by PEG-6000, and then, the physiological response of different cultivars under different degree of water stress were analyzed. The results showed that the content of MDA, proline and soluble protein, activities of SOD, POD and CAT changed defferenrly with different water stress. Under light water stress ( 10 % PEG ), the content of soluble protein increased, a great quantity of proline were accumulated, content of MDA was increased steeply, the activity of SOD, POD and CAT ascended; under heavy water stress ( 20 % PEG ) all these physiological indexes decreased or increased. Different cultivars expressed different sensitivity to water stress, this was related to their different capability of drought resistance.

【基金】 甘肃省科技厅(编号:Q5031-C31-27)
  • 【文献出处】 甘肃农业大学学报 ,Journal of Gansu Agricultural University , 编辑部邮箱 ,2005年03期
  • 【分类号】S532
  • 【被引频次】69
  • 【下载频次】452
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