【摘要】 以KRL-1(库尔勒香梨分离物)总RNA为模板,采用RT-PCR技术,扩增外壳蛋白(CP)基因的cDNA片段,将其克隆到pMD18-T载体上。通过序列测定,分析结果表明:KRL-1CP基因由714个核苷酸组成,编码237个氨基酸。KRL-1与ASGV其它分离物CP基因的核苷酸同源性为90%-93%,氨基酸同源性为94%-98%。根据KRL-1与其他分离物CP基因序列和生物学症状上的差异,可推断KRL-1存在较大的分子变异。更多还原

【Abstract】 Kuala pear virus isolate (KRL-1) which was originally obtained from Kuala pear’s petal by DU Yejuan was identified as Apple stem grooving virus (ASGV) by host range, ELISA, electron microscopy, and RT-PCR. However, KRL-1 was obviously different from other ASGV isolates in biological symptoms, so the coat protein gene (CP) of KRL-1 was cloned and sequenced. The sequence analysis showed that the capsid protein gene of KRL-1 contained 714 nucleotides, encoding a protein of 237 amino acids. The nucleotide and amino acid sequence of CP gene of this isolate shared 90% to 93% and 94% to 98% similarities to that of other ASGV isolates, respectively. The divergence of CP gene sequences and biological symptoms indicated that there was much molecular variability on KRL-1.更多还原