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苹果多酚提取物抗氧化活性的体外试验

Studies on antioxidant activities of apple polyphenol extracts in vitro

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【作者】 刘杰超王思新焦中高马彩红王亚绿郭焕霞王晓燕李银杰

【Author】 LIU Jie-chao,WANG Si-xin, JIAO Zhong-gao, MA Cai-hong, WANG Ya-lu, GUO Huan-xia, WANG Xiao-yan, LI Yin-jie (Zhengzhou Fruit Research Institute ,CAAS,Zhenzhou,Henan 450009 China;Sanmenxia Lakeside Fruit Juice Co.LTD,Sanmenxia, Henan, 472000 China)

【机构】 中国农业科学院郑州果树研究所河南省三门峡湖滨果汁有限责任公司河南省三门峡湖滨果汁有限责任公司 郑州 450009郑州 450009河南三门峡 472000河南三门峡 472000

【摘要】 从还原能力、抗脂质过氧化和对不同体系产生的活性氧自由基清除效果等方面对苹果多酚提取物的抗氧化 活性进行了试验研究和评价。结果表明苹果多酚提取物具有较强的还原能力,对脂质过氧化、Fenton反应产生的羟 自由基和光照核黄素及邻苯三酚自氧化产生的超氧阴离子自由基均具有很强的抑制作用或清除作用,且在试验浓 度范围内其最大抑制率或清除率分别可达到94.4%、96.64%、95.19%和90.48%,优于同浓度维生素C的效果或基本 相当。这表明该苹果多酚提取物是一种优良的天然抗氧化剂和自由基清除剂。

【Abstract】 The antioxidant activities of apple polyphenol extracts were studied and evaluated in vitro by determining the reducing capacity, antioxidant activity on lipid-peroxidation, and scavenging effects on active oxygen radicals generated by various system. The results showed that apple polyphenol extracts had stronger reducing capacity, antioxidant activity on lipid-peroxidation, and scavenging effects on hydroxyl radical and superoxide anion radical than ascorbic acid at the same concentration. In the range of test concentration, the maximum scavenging or inhibition rate of apple polyphenol extracts to linoleic acid auto-oxidation, hydroxyl radical generated by Fenton reaction, superoxide anion radical generated by illuminating riboflavin and pyrogallol auto-oxidation system was 94.4%, 96.64%, 95.19% and 90.48% respectively. The results suggested that the apple polyphenol extracts was an excellent natural antioxidant and free radicals scavenger.

【基金】 河南省重点科技攻关计划资助项目(0123014100)。
  • 【文献出处】 果树学报 ,Journal of Fruit Science , 编辑部邮箱 ,2005年02期
  • 【分类号】S661.1
  • 【被引频次】141
  • 【下载频次】1521
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