【摘要】 目的　探讨孕妇感染乙型肝炎病毒 (HBV)的状态与宫内感染的关系。方法　采用酶联免疫吸附(ELISA)及荧光定量PCR法对产前孕妇进行乙型肝炎两对半免疫学标志联合测定和HBVDNA载量检测 ;新生儿分娩后取脐动脉血检测HBVDNA载量。根据上述检测结果研究孕妇感染HBV的状态与宫内感染的关系。结果乙型肝炎e抗原 (HBeAg)和HBVDNA阳性的孕妇发生宫内感染率分别为4 6 .0 3% ,90 .4 8% ;HBeAg和HBVDNA阴性孕妇为 3.97% ,32 .5 4 % ;两组孕妇宫内感染率和HBVDNA阳性率比较 ,差异均有高度显著性 (P <0 .0 1)。孕妇血清中不同载量HBVDNA与新生儿宫内感染率进行直线回归分析 ,相关系数为 0 .96 73,成正相关。结论　以HBVDNA的载量作为观察指标研究分析孕妇HBV感染状态与宫内垂直传播的关系较免疫学指标HBeAg更加直接可靠 ;孕妇血清HBVDNA载量多是造成宫内感染的主要因素之一。更多还原

【Abstract】 Objective To evaluate the relationship between the hepatitis B markers and intrauterine infection in pregnant women. Methods Hepatitis B virus (HBV) markers and HBV DNA loading in pregnant women were detected by ELISA and fluorescence quantitative PCR methods respectively. HBV DNA loading in newborns’ umbilical blood was detected. Results 46.03% of pregnant women with positive HBeAg and 90.48% with positive HBV DNA had intrauterine infection respectively; 3.97% of pregnant women with negative HBeAg and 32.54% with negative HBV DNA had intrauterine infection respectively, there was significant difference in intrauterine infection and the positive rate of HBV DNA between two groups of women (P< 0.01). HBV DNA loading was positively correlated with the occurrence of intrauterine infection. Conclusion The detection of HBV DNA loading for analyzing the relationship between the hepatitis B and intrauterine infection in pregnant women is more reliable than the detection of HBeAg, the overloading of HBV DNA is one of the major factors for intrauterine infection.更多还原