【摘要】 目的比较中国健康汉族人和维吾尔族人GSTM1、GSTT1和GSTP1基因多态性分布。方法用多重PCR分析GSTM1和GSTT1基因多态性,PCR-RFLP检测GSTP15号外显子105位密码子基因多态性。结果汉族人与维吾尔族人的GSTM1纯合缺失频率接近,分别为56.1%和53.2%。而汉族人的GSTT1纯合缺失频率(50.0%)较维吾尔族人(26.6%)高。汉族人GSTP1105I/I、I/V和V/V基因型频率分别为60.7%,35.2%和4.1%;维吾尔族人分别为51.3%,40.2%and8.4%。结论维吾尔族人与汉族人,除GSTM1外,GSTT1与GSTP1突变基因型频率存在明显种族差异。更多还原

【Abstract】 Objective To compare the frequencies of polymorphisms of GSTM1, GSTT1, and GSTP1 in Chinese Uygur with those in Chinese Han. Methods GSTM1 and GSTT1 polymorphisms were analyzed by a polymerase chain reaction(PCR)-multiplex procedure, whereas GSTP1 polymorphism was analyzed by PCR-restriction fragment length polymorphism. Results The frequency of GSTM1 null genotype was 56.1% in Chinese Han and 53.2% in Chinese Uygur, respectively. Whilst the frequency of GSTT1 null genotype in Chinese Han (50.0%) was higher than that of Chinese Uygur (26.6 %). The genotype distribution of GSTP1 was 60.7% I/I, 35.2% I/V and 4.1% V/V in Chinese Han and 51.3% I/I, 40.2% I/V and 8.4% V/V in Chinese Uygur, respectively. Conclusion There is marked ethnic difference in the mutant frequencies of GSTT1 and GSTP1, but not GSTM1, between Chinese Uygur and Chinese Han.更多还原