【摘要】 用紫外光谱方法研究了四环素(TC) Cu(II)配合物与DNA的相互作用.吸收光谱研究表明,DNA能与四环素(TC)及Cu(II)形成的配合物发生反应,配合物与DNA的作用方式随着配合物类型及DNA浓度的不同而不尽相同:当四环素与铜形成1∶1型配合物时,较低浓度的DNA能与配合物以嵌插方式相互作用,而较高浓度的DNA与该配合物除了发生嵌插作用外,还存在另外的作用方式;当四环素与铜形成1∶2型配合物时,DNA与该配合物则主要以嵌插方式相互作用,并且这两种配合物与DNA的嵌插作用均是通过四环素配体插入的.更多还原

【Abstract】 The interaction of tetracycline(TC)-Cu(II) complex with DNA was studied with UV-Vis absorption spectrometry.The absorption spectrum studies indicate that DNA can interact with tetracyline(TC)-Cu(II) complex.The way of interaction between the complex and DNA varies in accordance with the types of complex and the concentration of DNA.If tetracycline interact with Cu(II) to form 1∶1 complex, the complex can interact with DNA by way of intercalation when the concentration of DNA is lower; when the concentration of DNA is higher,in addition to interacting with DNA by way of intercalation,the complex can interact with DNA by other way. If tetracycline interact with Cu(II) to form 1∶2 complex, the complex interact with DNA by way of intercalation mainly.That two kinds of the complex intercalate DNA all by way of tetracycline inserted in it.更多还原