【摘要】 目的探讨三苯氧胺(TAM)逆转卵巢癌细胞株的耐药性和逆转机制。方法应用ATP-TCA法检测细胞株的耐药性及TAM的逆转效果,应用流式细胞仪检测TAM对罗丹明123(Rh123)在细胞内积聚和外排的影响,应用免疫荧光技术定量测定TAM对P糖蛋白表达的影响。结果TAM能增加阿霉素对耐药株的细胞毒性作用,能增加耐药株细胞内Rh123的积聚,减少其外排,而对P糖蛋白的表达没有影响。结论TAM能部分逆转卵巢癌细胞株的耐药性,其强度与维拉帕米相当,其作用机理是抑制P糖蛋白的功能,而对其表达水平没有影响。更多还原

【Abstract】 Objective To investigate the reversal effect and the mechanism of tamoxifen(TAM) in ovarian carcinoma cell lines with multi-drug resistance(MDR). Methods The reversal efficacy of TAM were measured by ATP-TCA. The effects of TAM on Rh123 uptake and efflux were analyzed by flow cytometer. The effects of TAM on P-gp expression were analyzed quantitatively by immuno-fluorescence technique. Results TAM couldincrease the toxicity of adriamycin (ADM) in A2780/ADM, but not in A2780. TAM could increase cellular rhodamine 123(Rh123) accumulation and decrease its efflux, but had no effect on the expression of P-gp. Conclusions TAM can reverse MDR in ovarian carcinoma cell lines partly, and is similar to verapamil(VPL). TAM may act by blocking the function of P-gp and has no effect on the expression of P-gp.更多还原