【摘要】 目的:探讨PTEN mRNA 在兔晶状体上皮细胞增殖中的作用。方法: 将健康白色家兔42 只随机分为实验组(36只)和对照组(6 只)。实验组兔行双眼透明晶状体皮质吸除术,对照组未做处理。在术后1d ,3d,1wk,2wk,1mo 和2mo 各处死6 只。应用免疫组化法检测赤道部晶状体上皮细胞中增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA);应用原位杂交和RT-PCR 方法检测PTEN mRNA 的表达。结果: ①正常赤道部晶状体上皮细胞核可见PCNA弱阳性表达,术后1~7d PCNA 的表达维持在高水平状态。2wk后,随着时间的延长,PCNA 表达逐渐减弱,1～2mo 时大致恢复术前状态。②正常兔晶状体上皮细胞中PTEN mRNA 的表达呈阳性,术后1d 时PTENmRNA 表达明显下降;术后3d 时,PTEN mRNA 表达仍处于低水平状态。随着时间的延长,术后1wk 时略有恢复,2wk 时趋于明显好转,1 ～2mo 时恢复正常。PTEN mRNA 与PCNA 的表达呈负相关。结论:PTEN mRNA 在正常兔晶状体上皮细胞胞质中有表达;PTEN 参与晶状体上皮细胞的增殖代谢过程,对细胞的增殖起抑制作用。更多还原

【Abstract】 · AIM: To observe the effects of PTEN and the tumor suppressor gene in proliferative LECs of the rabbit.· METHODS: Forty-two white rabbits were randomly divided into test group (36 rabbits) and control group (6 rabbits). The transparent lenses of treated rabbits were operated with extra capsular cortex extraction, and the controls were kept untouched. The rabbits were sacrificed at 1d, 3d, 1wk, 2wK, 1mo and 2mo after surgery. Immunohistochemistry was applied to detect proliferative cellular nuclei antigen (PCNA) as well as hybridization in situ and reverse-transcriptase polymerase chain reactions (RT-PCR) were applied to detect phosphatase as well as tensin homology deleted on chromosome 10 (PTEN) mRNA in lens equartor.· RESULTS: PCNA expression increased significantly to a high level at 1wk, began to reduce 2wk later, and recovered to the normal level 1 or 2mo after the surgery. PTEN mRNA expressed positively in normal rabbit LECs. The relative PTEN mRNA contents reduced greatly at 1d after operation and remained low level at 3d. It began to increase slightly at 1wk, kept rising at 2wk and regained the normal expression after 1 or 2mo. There was inverse correlation between the PTEN mRNA and PCNA expression.· CONCLUSION: PTEN mRNA expresses positively in normal rabbit LECs plasma. PTEN participates in LECs proliferation and correlates with lens proliferative conditions. ·更多还原