【摘要】 目的探讨GSK3在动物肺组织及培养的猪支气管上皮细胞中的表达。方法用免疫组化,免疫细胞荧光法检测GSK3在人、大鼠、小鼠和猪的肺组织及培养的猪支气管上皮细胞中的表达。结果GSK3α和β广泛表达于人、鼠和猪的肺组织中,定位于胞浆。它们在几种动物肺组织中的表达分布大致相似,主要见于各级支气管上皮细胞、肺泡上皮细胞、粘膜平滑肌细胞和粘膜下腺体。但GSK3α在软骨细胞中表达明显强于GSK3β;几种哺乳动物肺组织中均未检测到GSK3的磷酸化。免疫荧光检测培养的猪支气管上皮细胞中有丰富的GSK3α、β的表达,磷酸化的GSK3信号弱。结论GSK3α、β丰富表达于不同种属哺乳动物肺组织的支气管上皮、腺体以及肺泡上皮,提示其可能在肺组织的生理功能和病理发生机制中起着重要作用。更多还原

【Abstract】 Objective To investigate the expression of glycogen synthase kinase 3(GSK3)in the lung tissues of human,rat,mouse and pig and in culturedpigbronchial epithelial cells.Methods The expression of GSK3 in lung tissue or cultured bronchial epithelial cells was observed by immunohistochemistry or immunofluorescencerespectively.Results Immunostaining showed that both GSK3αand GSK3βwere prominently expressed in the plasm of bronchial epithelial cells,submucosal glandcells,smooth muscle cells and alveolar epithelial cells of the above mammals.However,there were unexpected high levels of GSK3αin cartilage lacunacellswhen compared with those of GSK3β.No signals of phosphorylated GSK3α/βwere observed in pig the lung tissues .Immunofluorescencedemonstrated that the expressions of GSK3αand GSK3βwere high but phosphorylated GSK3α/βwas low in pig cultured bronchial epithelial cells.Conclusion The high and extensive expression of GSK3 in the major cells of the lung tissues of investigates mammals indicates that it may play an important role in the physiological and pathologicalprocesses of the lung.更多还原