【摘要】 利用正常细胞、凋亡细胞和坏死细胞在形态学特征上的差异,使用圆度和半径比率2个物理量来表征细胞活性状态,并运用微分干涉相衬(DIC)显微镜获得待测细胞图像,结合图像识别软件,提出了一种基于细胞形态学特征的细胞活性无损检测方法,测得了与细胞活性相关的有关参数。该方法应用于氨乙酰丙酸(ALA)的光动力疗法(PDT)后白血病细胞的活性检测得出的结论与使用四甲基偶氮唑盐比色(MTT)法检测得出的结论基本一致。该方法也可检测出死亡细胞中凋亡、坏死的比例;在对细胞测试的过程不需对细胞进行染色等预处理,是一种无损检测方法。更多还原

【Abstract】 Based on the morphologic distinctions of apoptosis from necrosis,by using two physical parameters,i.e.,roundness and radius ratio to present the viability of cells,a lossless method based on the differential interference contrast(DIC) image and identifying software,which is used to the viability of cells,is presented in the paper.By adjusting the area parameters of Jurkat cell,the viability of cells and the ratio of apoptosis to necrosis of are were estimated by the method.The experimental results are corresponding with that estimated by MTT method.更多还原