【摘要】 在2.2 mm内径的石英管中,采用正硅酸四乙酯水解的溶胶-凝胶法合成了填充细石英砂的高比表面积电色谱整体柱,并用正辛基三乙氧基硅烷键合制备反相色谱固定相.填充细石英砂的电色谱整体柱抑制了大柱径引起的电流热效应,采用电渗流驱动流动相,分离了苯酚和苯,实验证明该整体微柱用于电色谱分离和改善浓度检出限的可行性.更多还原

【Abstract】 Capillary electrochromatography(CEC) possesses advantages of high separation efficiency and selectivity,but it shows some shortcomings,such as low sample loadability and restricted concentration detectability,due to its small inner diameter.In this paper,a silica gel monolithic microcolumn with high surface area was prepared by sol-gel polymerization of tetraethoxysilane under acidic conditions,which was filled with fine quartz sand in 2.2 mm i.d.fused silica tube.The experiment results indicate that the heating effect caused by electric current in the large diameter microcolumn was eliminated by packing fine quartz sand.With the mobile phase driven by electroosmotic flow,two aromatic compounds were separated successfully by the reversed-phase electrochromatography.The experiment results indicate that the microcolumn can be used for electrochramatography separation and its detection sensitivity compared with CEC can be improved.更多还原