节点文献
无胶筛分芯片电泳研究贝类毒素-软骨藻酸与寡聚DNA的相互作用
Interaction Between Domoic Acid and Double-Stranded Oligodeoxy-nucleotide Studied by Using Microchip-based Non-gel Sieving Electrophoresis
【Abstract】 The interaction between domoic acid(DA) and 18-mer double-stranded DNA(dsDNA) was investigated by using microchip-based non-gel sieving electrophoresis. For the 18-mer dsDNA with a sequence of 3′-GCATTGGTTGACGTTGCA-5′, the amount of free dsDNA decreases with the increase of the added DA and also with the increase of reactive time. Meanwhile, the newly-produced two peaks were observed. The experimental results show that there is a strong interaction between DA and the 18-mer dsDNA. DA makes the degradation of the 18-mer dsDNA. The non-gel sieving electrophoresis on microchip is a rapid, convenient, highly sensitive method for studying the interaction between DNA and small molecules.
【关键词】 无胶筛分芯片电泳；
相互作用；
软骨藻酸；
双链DNA；
【Key words】 Microchip non-sieving electrophoresis； Interaction； Domoic acid； 18-mer dsDNA；
【Key words】 Microchip non-sieving electrophoresis； Interaction； Domoic acid； 18-mer dsDNA；
【基金】 国家自然科学基金(批准号:20275039);国家自然科学基金重点项目(批准号:20035010);国家自然科学基金重大项目(批准号:20299030);中国科学院仪器研制专项基金资助
- 【文献出处】 高等学校化学学报 ,Chemical Research In Chinese Universities , 编辑部邮箱 ,2005年07期
- 【分类号】R114;
- 【被引频次】6
- 【下载频次】137