【摘要】 采用质谱、圆二色谱、荧光光谱与细胞周期分析技术研究了重组内皮抑素的蛋白结构和对内皮细胞的作用机制.研究发现,以包涵体方式表达的蛋白中会有未被降解的N末端甲硫氨酸产物,重组内皮抑素在G2期阻断内皮细胞的生长.表明内皮抑素引发内皮细胞凋亡与细胞周期相关,为抗血管生成治疗肿瘤研究奠定了实验基础.更多还原

【Abstract】 The structure of recombinant endostatin and mechanism of acting on endothelial cell were studied by mass spectrometry, circular dichroism spectroscopy, fluorescence spectroscopy and cell cycle analysis technique. It was found that the recombinant protein prepared from inclusion body of E. coli may combinate the derivated protein with N-terminal Met. The mechanism by which recombinant endostatin causes G₂ arrest in endothelial cells was observed, which demonstrated that the apoptosis of endothelial cell induced by endostatin was correlated with cell cycle. The results lay the foundation for the antiangiogenesis therapy of the tumor.更多还原