【摘要】 通过3-含氧缩水甘油基-丙基-三甲氧基硅烷(GPTS)将二乙胺基乙基(DEAE)嫁接在介孔分子筛SBA-15的表面,制得弱阴离子交换层析剂DEAE-SBA-15,使其既具有分子筛作用,又具有离子交换作用的特点.经DEAE-SBA-15层析后,从粉尘螨代谢培养基中获得单一的洗脱峰,SDS-PAGE显示一条谱带,其相对分子质量为14000,表明经分离纯化所得到的蛋白质是粉尘螨主要变应原Derf2.对螨性哮喘患者皮肤点刺试验表明,所得Derf2仍具有变应原活性,且仅通过一次柱色谱即得Derf2,表明DEAE-SBA-15是一种较好的离子交换层析剂.更多还原

【Abstract】 Mesoporous silica SBA-15 was functionalized by 2-diethylamino-ethanol（DEAE） through the （epoxide） ring opening of 3-glycidyloxy-propyl-trimethoxy silane grafted on the silica surface（denoted as DEAE-SBA-15）. Subsequently, DEAE-SBA-15 was used as the weak anion exchange matrix for chromatography to purify （Der f 2） allergen from the spent culture medium extract of Dermatophagoides farinae, one of House dust mites that can cause heavy atopic diseases such as asthma and dermatitis. The structure of DEAE-SBA-15 was confirmed by （）¹³C and （）²⁹Si CP MAS NMR and other methods. By SDS-PAGE, Der f 2 was estimated to have a molecular weight of 14 000 and appeared as the only separated protein band. The assay results of skin prick test showed that the allergic activity of Der f 2 was retained. The above results indicate that DEAE-SBA-15 materials are promising fixed-matrix candidates in liquid chromatography to purify proteins.更多还原