【摘要】 在石英毛细管的进样端端口内采用相转移法制作聚砜膜 ,使该毛细管同时具有采样、样品净化与分离功能 .建立了一种透析 -毛细管电泳在柱联用方法 .膜能有效地拦截大分子和颗粒物 ,性能稳定 ,1 2 h内连续使用 ,膜的透过率和对大分子的拦截性能无显著变化 .该方法避免了大分子和颗粒物的干扰 ,可直接对复杂样品中的小分子进行分析 .将其应用于咖啡牛奶中游离咖啡因的含量测定 ,测得其值为 0 .68mmol/L;用于药物与蛋白的相互作用研究 ,获得了盐酸异丙嗪与牛血清白蛋白的结合常数 ,其值为 1 .47× 1 0 4 L/mol更多还原

【Abstract】 A novel method of in-line coupling of dialysis with capillary electrophoresis, which eliminates the influence of macromolecules and particles in sample matrixes during the analysis of small molecules, was established in this paper. A polysulfone membrane, which was prepared in the inlet end of the separation capillary by phase-inversion process, cut off macromolecules efficiently and durably, so the capillary can be used for clean-up sampling. Using this capillary in electrophoresis, little column efficiency lost was achieved and the RSD of migration times and peak areas of chlorpheniramine were 0.8% and 4.6%, respectively. During the continuous using of this capillary for 12 h, the performance of the membrane changed little. This technology was applied in the analysis of free caffeine in coffee milk, and with little interference, the concentration was determined to be 0.68 mmol/L. When this method was applied in interaction analysis, the binding constant of promethazine hydrochloride with bovine serum albumin was obtained, that is 1.47×104 L/mol.更多还原