【摘要】 数字共焦显微技术是一种新的共焦显微技术,其通过对三维样本显微图像的数字处理,获取高分辨率图像,无光漂白和毒化细胞现象,结构简单、价格便宜。简述了数字共焦显微技术及其进展,着重介绍了核心技术--去卷积算法及其特点:去模糊法速度很快,但易引入结构性假像,不宜用于测量和计算;线性复原法速度较快,复原效果较好,但会放大噪声和出现振铃;约束迭代法速度较慢,但复原能力强,分辨率高。各类算法在商业软件中都得到不同的应用。由于数字共焦显微技术的独有优势,随着去卷积算法的发展,数字共焦显微技术将获得更为广泛的应用。更多还原

【Abstract】 Digital confocal microscope technology (DCMT) is a new confocal microscopy. It can obtain high-resolution images by processing 3D light-microscopic images. It does not produce the light-bleach and envenom cell, and has simple machinery and low price. DCMT and its development, and the deconvolution algorithm-core of DCMT and its characteristic are introduced. Deblurring algorithms are extremely fast to compute, but may introduce structural artifacts, so it is not suitable for measurements and calculations; Linear algorithms have fast speed and good restoration effect, but may amplify noise and produce ringing; Constrained iterative algorithms are slow to compute, but have good restoring ability and high resolution. Along with the progress of algorithms, it is indicated that DCMT will be applied widely because of its peculiar advantages.更多还原