【摘要】 目的研究当归芍药散(DSS)抑制β-淀粉样蛋白(Aβ)沉积的作用。方法老化组单纯用Aβ1～42液,DSS组同时加入DSS,两组均37℃孵育箱体外孵育7d,运用透射电镜和硫磺素T荧光分析方法观察β-淀粉样蛋白Aβ1～42的聚集和纤维化形成的情况。结果电镜观察显示:老化组其纤维呈晶状聚集,长0.34～1.70μm,直径约10nm。DSS组Aβ聚集不明显,无明显Aβ纤维形成,主要表现为无定形结构。硫磺素T荧光分析:DSS组对Aβ纤维形成的抑制率为91.38%。结论提示DSS在体外有抑制Aβ聚集和纤维形成的作用。更多还原

【Abstract】 Objective To investigate if Danggui Shaoyao San could inhibit deposition of amyloid-β-protein ( Aβ) . Methods The solution in aging group was Aβ1-42 peptide. The solution in DSS group was Aβ1-42 peptide and DSS. The solution was incubated for 7 days in 2 group. The structure of Aβfibril was observed with transmission electron microscopy and thioflavine-T fluorometric assay. Results The solution in aging group showed a high density of fibril Aβaggregation and formation. However, only a few fibrils were found in DSS group by transmission electron microscopy. To compare with aging group, the Aβfibril formation and aggregation in the solution of DSS group was inhibited 91.38% by thioflavine-T fluorometric assay. Conclusion The results showed that DSS could inhibit Aβfibril formation and aggregation to a certain extent in vitro. It suggest that DSS may play a role in the drug therapeutic of AlzheimerWs disease by interfereing with the deposition of amyloid-β-protein.更多还原