【摘要】 RNA干扰(RNAinterference,RNAi),即用二十多个核苷酸组成的短的双链RNA(dsRNA)代替传统反义核酸进行转录后基因沉默,已经迅速而广泛地应用到基因功能,基因表达调控机制研究等热门领域。本文概述了RNAi的作用机制和近年来的研究进展,同时介绍了RNAi在植物分子生物学中的应用情况,主要包括植物基因功能的研究,植物的品质改良和植物中的转录后基因沉默(PTGS)。更多还原

【Abstract】 RNAi (RNA interference) refers to the introduction of homologous double stranded RNA (dsRNA) to specifically target a gene’s product, resulting in null or hypomorphic phenotypes. It has been applicated in many fields such as gene function and gene expression and regulation widely and rapidly. In this paper we reviewed the mechanism of RNAi and the advances in RNAi studies in recent years. Also, we reviewed the application of RNAi in plant molecular biology including gene function studies in plant, quality betterment studies in plant and post-transcriptional gene silencing (PTGS) in plant.更多还原