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山东小麦品种中矮秆基因Rht-B1b、Rht-D1b分布的分子鉴定

Distribution of Rht-B1b and Rht-D1b in Wheat Cultivars in Shandong Detected by Molecular Markers

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【Author】 Mu Meicai1,2 Liu Yong1 Guo Xiaoli3 Zhang Yueqiu2 Yu Kai 4 Liu Dongcheng1 Zhang Aimin1*1 Institute of Genetics and Developmental Biology, CAS, Beijing, 100101; 2 Yantai Agricultural School, Yantai, 264002; 3 China Agricultural University, Beijing, 100094; 4 Yantai Agricultural Technology Extension Center, Yantai, 264001

【机构】中国科学院遗传与发育生物学研究所,中国科学院遗传与发育生物学研究所,中国农业大学,山东省烟台农业学校,山东省烟台农技推广中心,中国科学院遗传与发育生物学研究所,中国科学院遗传与发育生物学研究所北京,100101；山东省烟台农业学校,烟台,264002,北京,100101,北京,100094,烟台,264002,烟台,264001,北京,100101,北京,100101；

【摘要】利用小麦矮秆基因Rht-B1b、Rht-D1b的4对特异分子标记,NH-BF和MR1、NH-BF和WR1.2、DF和MR2、DF和WR2,对山东小麦品种中矮秆基因Rht-B1b及Rht-D1b的分布情况进行了分子标记鉴定,结果表明在鉴定的150个品种中有20.67%含有Rht-B1b基因,有54.00%含有Rht-D1b基因,二者合计为74.67%;同时含有Rht-B1b和Rht-D1b2个矮秆基因的仅占3.33%。表明山东小麦矮化育种中Rht-D1b基因的利用远高于Rht-B1b。更多还原

【Abstract】 Four pair molecular markers, NH-BF and MR1, NH-BF and WR1.2, DF and MR2, and DF and WR2, specific for the discrimination between mutant (dwarf) Rht-B1b and Rht-D1b and their wild-type alleles, are used for study the distribution of Rht-B1b, Rht-D1b in the varieties of Shandong province. The screening of 150 cultivars reveals that 20.67% and 54.0% contains Rht-B1b, or Rht-D1b, respectively. Even though up to 74.67% contains one of the Rht-B1b and Rht-D1b dwarf genes, only 3.33% contains both of them. Our result clearly indicates that the Rht-D1b is more widely adopted in the dwarfing breeding of Shandong province.更多还原

【关键词】冬小麦；矮秆基因；分子标记； Rht-B1b； Rht-D1b；
【Key words】 Winter wheat； Dwarfing genes； Molecular marker； Rht-B1b； Rht-D1b；

【文献出处】分子植物育种 ,Molecular Plant Breeding , 编辑部邮箱 ,2005年04期

【分类号】S512.1
【被引频次】43
【下载频次】272

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