【摘要】 RFLP技术是一种高效的基因组DNA多态性分析技术,其原理是用限制性内切酶切割不同个体基因组DNA后,检测含同源序列的酶切片段在长度上的差异,具有可靠性较高,但操作烦琐、信息含量低等特点。本文综述了RFLP分子标记技术在蔬菜分类学及遗传多样性研究、遗传图谱构建、基因定位、分子标记辅助育种方面的应用情况,并对其应用前景进行了展望。更多还原

【Abstract】 Restriction fragment length polymorphism, that is briefly called RFLP, is a high-efficiency technology for the polymorphism of genome DNA analysis. RFLP was that deavage individuals DNA with restriction enzyme could make different length fragments of resistance gene aralogs, with more reliability, complicated manipulation and lower information. This paper reviewed the recent advance of the application of RFLP in various aspects of vegetable researches including genetic map construction, gene location and markers assisted selection.更多还原