【摘要】 目的建立一种用于高精确灵敏度分析的微量DNA样本制备方法。方法用显微捕获技术制备以细胞为单位的DNA样本,并用于ProfilerPlus试剂盒及ABI310遗传分析仪的灵敏度测试。结果建立了单细胞捕获操作方案,成功制备了依次含1～11个细胞的一组DNA分析样本,并可准确用于ProfilerPlus誖试剂盒及ABI310遗传分析仪的灵敏度研究。结论基于显微操作技术的高精确微量DNA分析样本制备方法可准确用于分析技术方法的灵敏度研究更多还原

【Abstract】 Objective To establish a reliable, exact and practical method to prepare DNA samples for sensitivity-test purposes. Methods The micromanipulation method was employed to prepare exact quantity DNA samples used to study the sensitivity of Profiler Plus?誖Kit-ABI PRISMTM 310 system. Results We succeed in establishing a micromanipulation method to prepare groups of DNA samples ,which contain 1-11 cells in turn, and also succeed in using them to study the sensitivity of Profiler Plus?誖Kit-ABI 310 system. Conclusion The method we have established is proved to be a reliable, exact and practical way to prepare DNA samples for sensitivity-test purposes.更多还原