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次甲基蓝褪色光度法测定脱氧核糖核酸
Determination of Deoxyribonucleic Acid with Methylene Blue by Spectrophotometry
【摘要】 研究了碱性染料次甲基蓝与脱氧核糖核酸的结合反应。在pH8.0~8.5的三羟甲基氨基甲烷-HCl介质中,次甲基蓝于630nm处有最大吸收,随着脱氧核糖核酸加入量的增加,其在630nm处的吸光度显著下降,下降程度与脱氧核糖核酸的量成正比。据此,建立了测定脱氧核糖核酸的新方法,方法的线性范围为0~7mg/L。该方法可用于合成样品分析。
【Abstract】 The binding reaction of deoxyribonucleic acid with methylene blue has been studied. In a pH range of 8.0~8.5, the system shows an absorption peak (630 nm), and this peak decreases with the increasing deoxyribonucleic acid concentration. Based on this, a new method for the determination of deoxyribonucleic acid at 630 nm using methylene blue as a labeling agent has been developed, and the linear range of determination is 0~7 mg/L. The method has been applied to the determination of deoxyribonucleic acid in synthetic samples with satisfactory results.
【Key words】 Deoxyribonucleic acid; Methylene blue; Fading function; Spectrophotometry;
- 【文献出处】 分析试验室 ,Analytical Laboratory , 编辑部邮箱 ,2005年06期
- 【分类号】Q523
- 【被引频次】3
- 【下载频次】156