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四磺化铁酞菁-阿尔新蓝8GX-对-羟基苯乙酸-H₂O₂催化荧光反应体系在核酸定量中的应用

Determination of nucleic acids based on the FeTSPc-Alcian blue 8GX-p-HPA-H₂O₂ catalytic fluorogenic reaction system

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【Author】 WEI Ling (Changji University, Changji 831100) and XU Jin-gou (Department of Chemistry, Xiamen University, Xiamen 361005), Fenxi Shiyanshi, 2005, 24(4): 37～39

【机构】新疆昌吉学院；厦门大学化学系昌吉831100；厦门361005；

【摘要】阳离子荧光染料阿尔新蓝 8GX与阴离子荧光染料四磺化铁酞菁发生缔合作用 ,使四磺化铁酞菁的荧光猝灭 ,当核酸存在时 ,削弱了离子缔合作用使四磺化铁酞菁的荧光恢复。荧光增强的程度与核酸的量成线性关系 ,据此实现了对核酸的定量测定更多还原

【Abstract】 Based on the ability of nucleic acids to shift the association equilibrium of an ion-association complex of alcian blue and iron-tetrasulfonatophthalocyanine, thus leading to an increase in phthalocyanine fluorescence, and the fluorescence intensity is linear correlating with nucleic acids contents, a new method was suggested for the fluorimetrc determination of nucleic acids.更多还原