【摘要】 在自行设计和制作的有机玻璃 (PMMA)微流控芯片上 ,以流动注射方式进样 ,利用对苯二酚对Luminol H2 O2 Co2 +体系化学发光 (CL)的强抑制作用 ,建立了间接测定对苯二酚含量的微型全化学分析系统 ( μ TAS)。对影响化学发光反应的各因素进行优化 ,对芯片结构的设计和反应过程进行探讨 ,设计并制作出适合该反应体系的芯片。在合成水样对苯二酚的测定中 ,化学发光强度的变化与对苯二酚的浓度在 1 .0× 1 0 - 7～ 2 .0× 1 0 - 8mol/L范围内成良好线性关系 ,检出限达 2 .0× 1 0 - 9mol/L。更多还原

【Abstract】 The miniaturized total analytical system （μTAS） which indirectly detected the concentration of hydroquinone was established by means of flow injection analysis （FIA） technique and the inhibited reaction of hydroquinone to Luminol-H₂O₂-Co²⁺ chemiluminescence on designed PMMA microchip. Different factors affecting the inhibited CL reaction, especially concentration of hydroquinone, were optimized. In order to design a microchip suitable to this reaction system, the design of chip configuration and the process of these reactions were studied. The chemiluminescence intensity had a linear relation ship with the concentration of hydroquinone in water samples, and the detection limit of this method was 2.0×10^-9 mol/L and the linear range was between 2.0×10^-7 and 2.0×10^-8 mol/L.更多还原