【摘要】 采用分光光度法研究了酸性棕NR与血清白蛋白的结合反应。在Brit ton Robinson(B-R)(pH2.50)缓冲溶液中,酸性棕NR与血清白蛋白结合生成沉淀,探讨了该结合反应的最佳条件,并据此建立了一种高灵敏度的测定血清白蛋白的新方法。牛血清白蛋白(BSA)和人血清白蛋白(HSA)分别在28.0～112.0mg/L、24.4～122.0mg/L范围内服从比尔定律,其表观摩尔吸光系数分别为:1 44×106L·mol-1·cm-1(BSA)、1.32×106L·mol-1·cm-1(HSA)。对7个人血清样品蛋白总量平行测定6次,相对标准偏差0.83%～3.02%,回收率90.90%～109.80%,且与医院双缩脲法结果基本一致。更多还原

【Abstract】 Acid brown NR （ANR） could combine with albumin such as bovine serum albumin （BSA） or human serum albumin （HSA） in acidic Britton-Robinson solution and produce a undissolved precipitation. Based on the decrease of ANR absorbance, a new method for determination of albumin was developed by spectrophotometry. Under the optimum reaction conditions, the linear ranges for the determination of BSA and HSA were 28.0～112.0 and 24.4～122.0 mg/L with absorption coefficient ε_BSA=1.4×10⁶ L·mol^-1 ·cm^-1, ε_HSA=1.3×10⁶ L·mol^-1·cm^-1, respectively. The relatively standard deviation and the recovery for the determination of total proteins in 7 human serum samples were 0.83%～3.02% and 90.90%～109.80%, respectively. The results of the determination were in agreement with that by Biuret method.更多还原