【摘要】 本文研究了家蝇幼虫抗菌肽MDL-1的荧光光谱和淬灭剂对内源性荧光的影响。家蝇幼虫抗菌肽MDL-1在激发波长280nm时,其荧光光谱为酪氨酸(Tyr)残基和色氨酸(Trp)残基共同提供。结果表明,KI不能淬灭抗菌肽MDL-1的Trp残基的荧光,而丙烯酰胺(Acr)能淬灭几乎所有的Trp残基的荧光(f=0.92);这说明,Trp残基不是位于抗菌肽分子的表面,而是位于分子的内部。更多还原

【Abstract】 Effects of quenching agents on the intrinsic fluorescence and fluorescence spectroscopy of the antibacterial peptide MDL-1 from Musua Domestica Larvae were studied.The results showed that fluorescence spectra of MDL-1 exhibited characteristic fluorescence of Trp residues and Tyr residuces at 280 nm.The Trp residue fluorescence of MDL-1 was not quenched by KI,but quenched by acrylamide. The fraction of Trp quenched obtained by the modified Stern-Volmer equation was 0.92.The Trp residues of MDL-1 were located in the inner district with negative dielectric instead of the surface.更多还原