【摘要】 通过固相萃取富集伏马菌素,采用液相色谱-电喷雾串联质谱法测定玉米和芦笋中的伏马菌素B1(FB1)、伏马菌素B2(FB2)。在最佳色谱条件下,伏马菌素B1、伏马菌素B2的保留时间分别为5.2 m in和8.8m in,样品空白无干扰。离子化技术采用电喷雾正离子方式,以多反应监测(MRM)方式进行检测。该法检出限为80 pg;线性定量范围为0.01~10mg/L;回收率为78.3%~104.9%;相对标准偏差为2.2%~14.2%。利用ESI/MS/MS对伏马菌素B1、伏马菌素B2进行了质谱解析,分别选择特征离子峰m/z352、528和m/z336、512作为伏马菌素B1、伏马菌素B2准确定性的依据。方法灵敏度高,选择性好,准确度高,可为食品中伏马菌素的风险评估提供灵敏、准确的分析方法。更多还原

【Abstract】 High performance liquid chromatography-electrospray ionization tandem mass spectrometry（ESI/MS/MS） combining with solid phase extraction has been developed for the determination of fumonisin B₁（FB₁） and fumonisin B₂（FB₂） in corn and asparagus.The gradient program was used to obtain an optimal separation with mobile phase of MeOH∶H₂O∶trifluoroacetic acid at 0.8 mL/min on a C₈ column.The retention time of FB₁ and FB₂ were 5.2 min and 8.8 min,respectively.Ionization was performed in an electrospray-（positive） ion source（+） and quantitative detection was carried out using Multiple Reaction Monitor mode.The limit of detection was 80 pg and the linear range was from 0.01 to 10 mg/L with recovery of 78.3%～104.9% and the RSD from 2.2 to 14.2%.Using the proposed ESI/MS/MS,FB₁ and FB₂ could be identified with both retention time and the typical ion peaks for FB₁ at m/z 532 and 528 and FB₂ at m/z 336,512,（respectively）.The method was proved to be validated to analyze these two fumonisins from asparagus and corn samples.更多还原