【摘要】 建立了可用于快速、灵敏地检测双酚A的生物素-亲和素放大酶联免疫吸附测定法,测得最佳实验条件为包被抗原浓度为13.8mg/L、抗体稀释为2.4×105倍,生物素化二抗和酶标亲和素的最佳稀释倍数分别为2000倍和500倍。在优化条件下,方法的线性范围0.2~1000μg/L;最低检出限0.05μg/L。所建立的方法用于食品和唾液中双酚A含量的测定,样品处理简单,结果满意。更多还原

【Abstract】 A rapid and sensitive method based on biotin-avidin amplified enzyme linked immunosorbent assay (ELISA) for the determination of bisphenol A (BPA) was established. The optimal concentration of coated antigen was found to be 13.8 mg/L. The dilution folds of the anti-BPA immunoglobulin G(IgG), biotinylated goat anti-rabbit IgG and avidin-horseradish peroxidase (HRP) were all optimized and the optimum results were found to be 2.410~5, 2000 and 500, respectively. To prevent the possible nonspecific adsorption of biotinylated goat anti-rabbit IgG and avidin-HRP, 0.01 mol/L phosphoat buffer solution (PBS) containing 10% fetal bovine serum was used as the diluting solvents. The linear range of the presented method was from 0.2 to 1000 μg/L, and the limit of detection was found to be 0.05 μg/L. The method has been applied to determine bisphenol A in canned corn and saliva sample with satisfactory results.更多还原