【摘要】 应用毛细管电泳电化学检测方法,以金属铜电极为检测电极,研究了同时测定人血浆中水溶性小分子抗氧化剂谷胱甘肽(GSH)、尿酸(UA)、色氨酸(Try)和半胱氨酸(Cys)的最佳条件。在最佳实验条件下,各组分的线性范围在1.0×10-6～5.0×10-4mol/L内;检出限在10-6mol/L数量级。该法具有分析速度快、灵敏度高等优点,对血浆样品的测定取得了满意的结果。更多还原

【Abstract】 [WT]The watersoluble small molecular antioxidants including glutathione （GSH）, uric acid （UA）, tryptophan （Try） and eystein （Cys） are very important to human being health and their detection is significant too. Because they are electroactive, electrochemical detection is more effective than ultraviolet detection in their analysis and performs a higher sensitivity. As a powerful separation technique, capillary electrophoresis has some advantages such as cheap instrumentation, low sampling volume and high speed and has been extensive studied and applied in biological analysis in past twenty years. The optimum conditions of determining GSH, UA, Try and Cys by capillary zone electrophoresis with electrochemical detection （CZE-AD） based on metallic copper electrode were studied in detail. The optimum conditions of CZE-AD were separation voltage 15 kV, running buffer 25 mmol/L Na2B4O750 mol/L NaH₂PO₄（pH 7.9）, kinetic injection time 30 s and detection potential 0.5 V. The linear ranges of the four analytes were（1.0⁵.0）×10 -6 ⁵.0×10 -4 mol/L and the limits of detection were about 10 -6 mol/L under the optimal experimental conditions. This method performed some advantages in quickness, high sensitivity and simple instrumentation, and satisfactory results were obtained when it was applied to analyzing human plasma samples.更多还原