【摘要】 目的:研究退变腰椎间盘组织胶原含量的变化对MRI信号强度的影响。方法:28例患者行腰椎间盘MRI扫描,依据T2WI信号强度对其退变程度分为三类;测量拟手术椎间盘及脑脊液T2WI信号强度,计算出二者的比值。全部患者行PLD手术,取出的30个髓核标本行Ⅰ、Ⅱ型胶原的免疫组化染色,并测定染色区的平均光密度以及半定量胶原含量。结果:全部髓核组织中均有Ⅰ、Ⅱ型胶原的阳性染色,Ⅱ、Ⅲ类椎间盘胶原染色强度分别为0.50±0.08、0.54±0.06,二者之间差异有显著性意义(t=-2.51,P=0.02)。胶原染色总强度与平均信号强度比值之间的相关系数为-0.73(P<0.001)。结论:腰椎间盘退变时,胶原含量的增多是引起椎间盘T2WI信号强度降低的生物化学基础之一。更多还原

【Abstract】 Objective:To study change of collagen content cause the influence to MRI signal intensity （SI） of lumbar intervertebral disc.Methods:28 cases with lumbar intervertebral disc herniation received MRI examination.All discs were classified into three categories according to their SI in T₂WI image.After SI of disc and cerebrospinal fluid SI were measured,the ratio of disc SI to cerebrospinal fluid SI was calculated.All cases received PLD procedure and 30 specimens of lumbar intervertebral disc were got.The type Ⅰ and Ⅱ,collagen in the specimens were stained by the method of immunohistochemical method.The optical density means of positive staining areas were measured to represent for the collagen content.Results:There existed positive staining areas of Ⅰ,Ⅱ type,collagen in all specimens.The optical density means of Ⅱ,Ⅲ type,collagen were respectively 0.4798±0.0783、0.5421±0.0579.There was statistically significant difference between them （t=-2.509,P=0.018）.The correlative coefficient between SI ratio and collagen staining intensity was -0.728 （P=0.000）.Conclusion:Increasing of collagen content is one of biochemical backgrounds to cause the decreasing of disc T₂WI signal intensity when lumbar intervertebral disc degenerates.更多还原