【摘要】 目的:探讨血清标本的保存、标本溶血、脂浊血清、细菌污染等对β-AP检测结果的影响。方法:取9例血清标本置4℃保存,观察β-AP含量与保存期的关系;人为造成标本溶血观察β-AP含量;脂浊血清与脱脂血清β-AP含量比较;细菌污染血清标本后观察β-AP含量变化。结果:血清标本4℃贮存4dβ-AP结果无显著变化(P>0.05);溶血标本β-AP值明显增高(P<0.01);脂浊血清β-AP含量较脱脂血清高(P<0.05);细菌污染标本后对β-AP含量检测有影响。结论:β-AP在4℃保存4d含量稳定,在室温中迅速降解。标本溶血、脂浊、细菌污染均可致β-AP检测结果造成异常变化,在实际工作中必须予以重视。更多还原

【Abstract】 Objective To study the effect of ① storage time ② hemolysis ③ excessive lipid content ④ bacterial contamination on the tested serum β-AP RIA results. Methods ① Specimen was stored at 4℃ and β-AP contents determined daily for seven consecutive days (9 specimens). ② specimen was assayed, then the clot forcibly broken to cause hemolysis and the serum re-assayed (8 specimens). ③ Hyperlipemic specimen was assayed, the excess lipid extracted with ether and serum re-assayed (8 specimens). ④ Specimen was assayed and left at room temperature for a few days. The specimen was re-assayed for several times after bacterial growth (10 specimens). Results serum β-AP contents were stable up to 4 days under 4℃ (P>0.05). Serum β-AP contents increased significantly after hemolysis (P<0.01). β-AP contents in hyperlipemic samples decreased significantly after ether treatment (P<0.01). With bacterial growth, the β-AP contents could increase or decrease, depending upon the type of bacteria. Conclusion Serum β-AP was stable up to 4 days at 4℃, but rapid degraded under room temperature. Moreover, hemolysis, hyperlipemia and contamination would all affect the RIA result and should be carefully dealt with in daily work.更多还原