【摘要】 目的研究类风湿关节炎(rheumatoidarthritis,RA)患者病灶部位即关节滑膜组织浸润的受某种自身抗原驱动的T淋巴细胞抗原受体(Tcellreceptor,TCP)"链的取用格局。方法采用实时定量聚合酶链反应(polymerasechainreaction,PCR)的方法分析RA患者病灶部位T淋巴细胞抗原受体"链的取用;采用PCR-SSP方法对所调查的RA患者的人类白细胞抗原(humanleucocyteantigen,HLA)进行了分型。结果研究发现中国人群RA患者病灶关节滑膜浸润的T淋巴细胞抗原受体主要取用BV14和BV16家族;中国人群RA患者HLA基因型主要以HLADRB1*0405为主要特征,与白种人RA患者不同。结论研究提示中国人群RA患者病灶区自身反应性T细胞具有TCRBV14和BV16的限制性取用,其中TCRBV16的偏移为国内外首次报道。而HLADRB1*0405类风湿关节炎患者表现出TCRBV16取用的趋势,提示TCRBV16取用受主要组织相容性复合体(MHC)的约束,这为进一步分析诱发RA的发生的免疫异常和自身反应性T细胞的生物学特性提供了重要试验基础。更多还原

【Abstract】 Objective To identify T cell receptor (TCR) structural feature characteristics of infiltrating T cells derived from synovial fluid or synovial membrane of rheumatoid arthritis (RA) patients. Methods The BV gene distribution analysis was performed quantitatively in a cohort of Chinese RA patients using real-time polymerase chain reaction (PCR), and human leucocyte antigen (HLA) genotyping was performed by PCR-SSP. Results The results of the study revealed that infiltrating T cells derived from synovial lesion tissue of RA patients displayed marked skewing of BV gene distribution toward BV14 and BV16. HLA genotyping was most of DRB1*0405 in RA patients. Conclusion The finding supports the notion that HLA genotypes and racial background may influence BV skewing of synovial infiltrating T cells in RA and provides an explaination for characteristic over-expression of BV16 in this Chinese RA population. This study has further revealed a trend towards correlation of the over-expression of BV16 but not BV14 in synovial T cells and HLA DRB1*0405 in RA patients.更多还原