【摘要】 目的探讨IL-10受体(IL-10R)在系统性红斑狼疮(SLE)发病机制中的作用及其临床意义。方法应用反转录-聚合酶链反应(RT-PCR)检测89例SLE患者和30名正常人外周血单个核细胞(PBMC)中IL-10RmRNA的表达水平;用酶联免疫吸附试验(ELISA)法检测血清中IL-10水平。结果①活动期SLE患者和非活动期SLE患者及正常人IL-10受体表达阳性率为100%。②PBMC中,IL-10RmRNA表达水平:活动期SLE患者(1组)为0.87±0.29,非活动期SLE患者(2组)为0.72±0.21,正常人(3组)为0.68±0.14。1组和2组间比较差异有统计学意义(P<0.05),1组和3组间比较差异有统计学意义(P<0.05),2组和3组间比较差异无统计学意义(P>0.05)。③血清中IL-10水平:1组和2组间比较差异无统计学意义(P>0.05),1组显著高于3组(P<0.05),2组显著高于3组(P<0.05)。活动期SLE患者和非活动期SLE患者血清中的IL-10水平与PBMC上的IL-10R的表达水平呈正相关(r=0.888,r=0.787,P<0.05)。结论IL-10及其受体的异常表达可能在SLE疾病活动和发展过程中起重要作用。检测SLE患者PBMC中IL-10受体的表达水平可作为疾病的活动性指标之一。更多还原

【Abstract】 Objective To investigate the role of interleukin (IL)-10R in the pathogenesis of systemic lupus erythematosus (SLE) and its clinical significance. Methods RT-PCR technique was used to semiquantitatively analyze IL-10R mRNA expression in PBMC and double antibody sandwich ELISA was applied to determine IL-10 in serum from 89 patients with SLE. Results The positive IL-10R mRNA expression was found in all patients in the active SLE patient group (groupⅠ), the inactive SLE group (groupⅡ) and the control group (group Ⅲ). The mean levels of IL-10R mRNA expressions in groupⅠ,Ⅱ and Ⅲ were 0.87±0.29, 0.72±0.21 and 0.68±0.14, respectively. Compared with group Ⅱand groupⅢ, the mean level of IL-10R mRNA expressions in groupⅠ was markedly increased (P<0.05), while there was no statistical differences between groupⅡ and groupⅢ (P>0.05). The mean levels of IL-10 in serum of groupⅠ,Ⅱ and Ⅲ was 32.32±11.51, 18.66±6.63 and 0.289±0.867, respectively. Compared with group Ⅱand groupⅢ, the mean level of IL-10 of groupⅠ was significantly higher than those in groupⅡ and groupⅢ (P<0.05), while that in groupⅡwas higher than groupⅢ (P<0.05). There was positive correlation between IL-10 and IL-10R in active group and inactive group (r=0.888, and r=0.787, P<0.05). Conclusions The expression of IL-10R mRNA in PBMC and the level of IL-10 of serum in patients with active SLE are significantly higher in than that in patients with inactive SLE and control group. The level of IL-10 in patients with inactive SLE is higher in than control group. The abnormal expression of IL-10 and IL-10R may play an important role in the pathogenesis of SLE, and the levels of IL-10R may be used as one of the indices for active SLE.更多还原