【摘要】 目的探讨β-连环蛋白、细胞周期蛋白D1(Cyclin D1)在喉鳞癌的表达及其意义。方法采用免疫组织化学SP法检测58例喉鳞癌及10例正常喉上皮组织β-连环蛋白及Cyclin D1的表达。结果10例正常喉上皮β-连环蛋白表达位于细胞膜,细胞质未见表达;Cyclin D1均呈阴性表达。58例喉鳞癌组织中,31例β-连环蛋白细胞质表达,16例细胞核表达;34例Cyclin D1细胞核表达。pN+的β-连环蛋白细胞质表达阳性率明显高于pN0(P<0.05),T3~T4喉癌病例中β-连环蛋白细胞质表达阳性率明显高于T1~T2喉癌病例(P<0.05);β-连环蛋白细胞质阳性表达与喉鳞癌部位、分化程度无关(P>0.05)。Cyclin D1阳性表达仅与喉癌浸润程度相关(P<0.05),而与喉癌发生部位、分化程度、淋巴结转移无关(P>0.05)。31例β-连环蛋白细胞质表达喉癌组织中23例CyclinD1阳性表达,明显高于β-连环蛋白细胞膜表达者。结论喉鳞癌中β-连环蛋白表达可作为评估喉鳞癌恶性程度的重要生物学指标。更多还原

【Abstract】 Objective To study the expression pattern of β-catenin and cyclin D₁ in laryngeal squamous cell carcinoma（LSCC） tissue,and discuss its clinical and pathological significance.Methods By using immunohistochemical staining（SP method）,the expression of β-catenin and cyclin D₁ were detected in 10 laryngeal normal membrane and 58 LSCC.Results In laryngeal normal tissue,β-catenin showed high intense expression at the membrane and negative expression at the cytoplasm and nucleus.Cyclin D₁ showed negative expression at the nucleus.In contrast to the normal tissue,β-catenin was expressed variously in the cytoplasm in carcinoma but less,or even lost expression at the membrane in cancer samples.In 16 cases,β-catenin could be detected in the nucleus.the positive expression of cyclin D₁ was mainly confined to the nuclei（34/58）.Moreover,the positive rate of β-catenin cyclin D₁ expression in cytoplasm was significantly higher in those patients with lymph node metastasis and T_3-4 staging than patients with no lymph node metastasis and T_1-2（P<0.05）.But had no correlation with the tumor differentiation,location（P>0.05）.The positive expression of cyclinD₁ had only a correlation with invasive depth,but not correlated with the tumor differentiation,location,lymph node metastasis.In LSCC,the aberrant expression of β-catenin in cytoplasm and cyclin D₁ in nucleus was positively related to the expression of cyclin D₁（P<0.05）.Conclusion β-catenin and cyclin D₁ expression could be used as an important biological indices for evaluating the malignancy of LSCC.更多还原