A recombinant plasmid pQE30-bgln was constructed for obtaining the BGLN protein expressed in E.coil so as to develop the efficiency of extracting resveratrol from plants.Touch-down PCR was used to amplify the bgln gene from plasmid pUCP67 in which bgln was cloned.A new plasmid pQE30-bgln,was then constructed by inserting the amplified bgln gene into pQE30,a prokaryotic expression vector.Restriction analysis and sequencing were used to confirm the structure of pQE30-bgln.Results showed that a DNA fragment in...
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