【摘要】 背景与目的　生化及遗传学研究提示ras蛋白在细胞增殖分化信号从激活的跨膜受体传递到 下游蛋白激酶的过程中起重要作用。本研究拟探讨信号传导转录活化成员3(signaltransducerandactivator oftranscription3,STAT3)和有丝分裂激活的蛋白激酶(mitogenactivatedproteinkinases,ras MAPK)信号 传导通路中的ras和p38在非小细胞肺癌中的表达,研究STAT3与ras和p38的关系。方法　选取42例手 术切除非小细胞肺癌标本,包括癌组织和癌旁肺组织,应用Westernblot检测癌组织和癌旁肺组织的ras、p38 及STAT3蛋白含量;RT PCR方法检测ras不同表达的癌组织中p38和STAT3mRNA含量;应用免疫荧光 观察p38和STAT3在癌组织和癌旁肺组织中的分布差异。结果　ras、p38及STAT3在癌组织中蛋白相对 含量为0.6012、0.6724、0.5119,癌旁肺组织中为0.2793、0.3071、0.1917,癌组织中的表达均明显高于癌旁肺 组织(P<0.01);在ras蛋白含量高于平均值的肺癌组织中,p38及STAT3蛋白表达量为0.7624和0.6262, mRNA含量为1.0309和1.0538,均明显高于ras蛋白含量低于平均值的肺癌组织(0.4715和0.2569;0.6569 和0.3437,P<0.01)。ras的表达与p38和STAT3均明显相关(相关系数分别为0.809和0.842,P<0.01)。更多还原

【Abstract】 Background and objective Biochemical and genetic researches suggest that ras protein plays an important role in transduction process of cell proliferation differentiation signals from activated transmembrane receptors to substream protein kinases. This study is to explore the expression of ras, p38, both of which are members of MAPK (mitogen activated protein kinases) signal transduction pathway, and STAT3 (signal transducer and activator of transcription 3) in non-small cell lung cancer, and the association among them. Methods Forty-two resected lung cancer and paracancerous lung tissue samples were used to determine the protein expression of ras, p38 and STAT3 with Western blot, the mRNA expression of p38 and STAT3 in lung cancer tissues of various ras protein expression with RT-PCR. The location of p38 and STAT3 in lung cancer tissues was revealed with immunofluorescent staining. Results The relative protein expressions of ras, p38 and STAT3 were 0.6012, 0.6724, 0.5119 in cancer tissues, and 0.2793, 0.3071, 0.1917 in paracancerous lung tissues, respectively (P<0.01). The protein and mRNA expressions of p38 and STAT3 ( 0.7624 and 0.6262; 1.0309 and 1.0538) in cancer tissues with higher ras protein expression were remarkably higher than those with lower ras protein expression (0.4715 and 0.2569; 0. 6569 and 0.3437, P< 0.01). There was a significant correlation between the expression of ras, p38 and STAT3 (correlation coefficient: 0.809 and 0.842, P<0.01), and so was the p38 and STAT3 (correlation coefficient: 0.829, P<0.01). Conclusion Abnormal expressions of STAT3 and some factors of ras-MAPK signal transduction pathway exist in the oncogenesis and development of non-small cell lung cancer, and many of them may have crosstalk.更多还原