【摘要】 目的:研究哮喘大鼠肺组织中血红素氧合酶-1(HO-1)的表达情况及克拉霉素对HO-1的调控作用;观察其HO-1表达与BALF中EOS占细胞总数的百分比(EOS%)、嗜酸性粒细胞(EOS)、全血COHb的百分比含量之间的相关性。方法:清洁级雄性SD大鼠30只,随机分为正常对照组(N组)、哮喘组(A组)、克拉霉素治疗组(C组),每组10只。测定全血COHb的百分比含量;计数BALF沉渣中细胞总数和分类;计数肺组织中浸润的炎性细胞数;免疫组织化学染色法观察HO-1在哮喘大鼠肺组织的表达。结果:HO-1主要表达在气道上皮细胞,三组HO-1阳性表达的平均吸光度分别为0.07±0.01、0.22±0.03、0.14±0.02。A组HO-1表达水平显著高于N组(P<0.001),C组HO-1蛋白的表达显著低于A组(P<0.01)。HO-1表达水平与全血COHb的百分比含量、BALF中EOS占细胞总数的百分比及肺组织中EOS总数均呈显著正相关(分别为r=0.887,P<0.01;r=0.889,P<0.01;r=0.883,P<0.01)。结论:哮喘大鼠的肺组织HO-1表达水平显著增加,提示HO-1可能参与哮喘发病过程。克拉霉素明显改善哮喘的气道炎症浸润,其作用机制部分是通过抑制HO-1起作用。更多还原

【Abstract】 Objective: To investigate the protein expressions of HO-1 in the lung tissue of asthmatic rat and to study the regulation roleof clarithromycin on HO-1. To research whether the ratios of eosinophils to the total cell numbers (EOS%) in BALF, the numbers ofeosinophils in lung tissue (EOS), the percent content of blood carbon monoxide Hb (COHb) and the expressions of HO-1 has a correlationin the lung tissue of asthmatic rat. Methods: Thirty Sprague-Dawley (SD) male rats were randomly divided into 3 groups: the controlgroup (N group), asthma group (A group), CAM group (C group), each group had 10 rats. The assessment of the percent content of bloodcarbon monoxide Hb (COHb) was performed. The total cell number and differentiation cell numbers in bronchoalveolar lavage fluid(BALF) and the inflammatory cells of airway wall were observed, the protein expressions of HO-1 in airway wall was detected by immuno-histochemistry techniques. Results: The expression of HO-1 was mainly located in airway epithelium in the 3 groups (N, A, C) with theoptical densities being 0.07±0.01, 0.22±0.03, 0.14±0.02, respectively. The asthmatic group (group A) showed higher optical densities thanthat of the control group (group N) (P < 0.001), the protein expressions of HO-1 in airway wall of group C was significantly lower thanthose of group A (P < 0.01). There was a significant correlation between the expression of HO-1 in airway wall and the percent content ofblood carbon monoxide Hb (COHb) (r = 0.887, P < 0.01), EOS% in BALF (r = 0.889, P < 0.01), and the numbers of EOS in lung tissue(r = 0.883, P < 0.01). Conclusion: The expression of HO-1 was increased significantly in the lung tissue of asthmatic rat, suggesting HO-1 might play a significant role in the pathogenesis of asthma. CAM demonstrates anti-inflammatory effects partly by downregulating theexpressions of HO-1.更多还原