【摘要】 为了测定绵羊体内α甘露糖苷酶最适反应的pH,建立一种适合于绵羊血清α甘露糖苷酶活性的检测方法。根据对-硝基苯基-α-D-甘露糖苷在α甘露糖苷酶的作用下可水解产生对硝基酚和甘露的活性,用比色法测定产生的对硝基酚含量,与标准曲线对照,从而求出α甘露糖苷酶活性。试验对2份绵羊血清的α甘露糖苷酶活性在不同pH条件下进行了测定。研究表明,绵羊血清中α甘露糖苷酶在pH 3.8时活性比pH 4.6和pH5.0高,差异显著(P<0.05),初步推测绵羊体内α甘露糖苷酶最适反应的pH为3.8。更多还原

【Abstract】 To determine the optimum pH of α-mannosidase reaction from sheep,and establish a method of α-mannosidase activity test for sheep.By the products of reaction of p-nitrophenyl-α-D-mannopyranoside and α-Mannosidase is p-nitrophenol and mannose.The concentration of p-nitrophenol produced was estimated using the colorimetric procedure,and obtained α-mannosidase activity compared with the standard curve.The α-mannosidase activity of two serum from sheep was tested in different pH.Results showed that α-mannosidase activity at pH 3.8 was higher than at pH 4.6 and pH 5.0,the variance is significant(P<(0.05)),and primarily presumed that pH 3.8 was the optimum pH of the reaction of α-mannosidase from sheep.更多还原