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羊和猪胎盘肽的提取

Extration and Identification of Placental Peptide from Caprine and Swine

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【作者】 周国华孟凡军王永志葛利江

【Author】 ZHOU Guo-hua~(1),MENG Fan-jun~(2),WANG Yong-zhi~(3),GE Li-jiang~(1)(1.College of Veterinary Medicine,Shandong Agricultural University,Taian,Shandong,271018,China;2.Yishui Pasturage Bureau of Shandong,Yishui,Shandong,276400,China;3.Jinan Agricultural College,Shandong,Jinan,250023,China)

【机构】 山东农业大学动物科技学院山东省沂水县畜牧局济南市农业学校山东农业大学动物科技学院 山东泰安271018山东沂水276400山东济南250023山东泰安271018

【摘要】 利用超滤法从羊和猪的胎盘中提取胎盘肽,用改良的加脲Tricine-SDS-PAGE电泳法对其进行初步定性鉴定。成功分离显示出了羊的胎盘肽,其分子质量小于5 ku,与人胎盘肽的分子质量范围完全符合。猪的6 ku的蛋白带很明显,但低于5 ku的蛋白带不是很明显。

【Abstract】 The distilled caprine and swine placental peptide was prepared by ultrafilter and identified by improved Tricine-SDS-PAGE eletrophoresis with urea in the experiment.Caprine placental peptide was prepared and separated,which had no more than 5 ku of molecular weight,according with the molecular weight range of human placental peptide completely.As to swine placental peptide,the electrophoretic lane of 6 ku molecular weight was obvious,but less than 5 ku was obscure.

  • 【文献出处】 动物医学进展 ,Progress In Veterinary Medicine , 编辑部邮箱 ,2005年09期
  • 【分类号】S852.23
  • 【被引频次】15
  • 【下载频次】302
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